Journal: Molecular Medicine Reports

Article Title: Identification, characterization and microRNA expression profiling of side population cells in human oral squamous cell carcinoma Tca8113 cell lines

doi: 10.3892/mmr.2020.11073

Figure Lengend Snippet: Expression of stem genes detected by RT-qPCR and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.

Article Snippet: The RT reactions contained 1 µl RNA, 2 µl 5X PrimeScript Buffer, 0.5 µl PrimeScript RT Enzyme Mix, 0.5 µl Oligo dT Primer, 0.5 µl Random 6-mers and 5.5 µl RNase Free dH 2 O. qPCR was performed using a StepOne Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) with One Step SYBR PrimeScript RT-qPCR kit II (Takara Bio, Inc.).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction