5x primescript kit Search Results


5x primescript kit  (TaKaRa)
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TaKaRa 5x primescript kit
5x Primescript Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5x prime script rt kit  (TaKaRa)
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TaKaRa 5x prime script rt kit
5x Prime Script Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5x primescript reverse transcription reagents  (TaKaRa)
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5x Primescript Reverse Transcription Reagents, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent kit  (TaKaRa)
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TaKaRa reagent kit
Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5x primescript buffer takara clontech  (TaKaRa)
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5x primescript buffer  (TaKaRa)
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TaKaRa 5x primescript buffer
5x Primescript Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primescript reverse transcriptase rt kit  (TaKaRa)
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TaKaRa primescript reverse transcriptase rt kit
Primescript Reverse Transcriptase Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complementary dna cdna  (TaKaRa)
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one step sybr primescript rt qpcr kit ii  (TaKaRa)
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TaKaRa one step sybr primescript rt qpcr kit ii
Expression of stem genes detected by <t>RT-qPCR</t> and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.
One Step Sybr Primescript Rt Qpcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primescript  (TaKaRa)
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TaKaRa primescript
Expression of stem genes detected by <t>RT-qPCR</t> and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.
Primescript, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primescript 1st strand cdna synthesis kit  (TaKaRa)
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TaKaRa primescript 1st strand cdna synthesis kit
Expression of stem genes detected by <t>RT-qPCR</t> and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.
Primescript 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5x gdna eraser buffer  (TaKaRa)
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TaKaRa 5x gdna eraser buffer
Expression of stem genes detected by <t>RT-qPCR</t> and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.
5x Gdna Eraser Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of stem genes detected by RT-qPCR and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.

Journal: Molecular Medicine Reports

Article Title: Identification, characterization and microRNA expression profiling of side population cells in human oral squamous cell carcinoma Tca8113 cell lines

doi: 10.3892/mmr.2020.11073

Figure Lengend Snippet: Expression of stem genes detected by RT-qPCR and western blotting. (A) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells. (B) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells. (C) Expressions of ALDH1, Sox2, Oct4, Nanog and CD133 mRNA by RT-qPCR in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. (D) Expressions of ALDH1, Sox2, Oct4 and Nanog protein by western blotting in SP and non-SP cells following differentiation by culturing in serum-supplemented medium for 14 days. *P<0.05, **P<0.01 vs. respective non-SP. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ALDH1, aldehyde dehydrogenase-1; Sox2, SRY-box 2; Oct4, POU class 5 homeobox 1; Nanog, nanog homeobox; CD133, CD133 antigen; SP, side population.

Article Snippet: The RT reactions contained 1 µl RNA, 2 µl 5X PrimeScript Buffer, 0.5 µl PrimeScript RT Enzyme Mix, 0.5 µl Oligo dT Primer, 0.5 µl Random 6-mers and 5.5 µl RNase Free dH 2 O. qPCR was performed using a StepOne Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) with One Step SYBR PrimeScript RT-qPCR kit II (Takara Bio, Inc.).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction